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SNAKES, COLUBRID, NEW WORLD

Classification   |    Detailed evidence-based information

Therapeutic Toxic Class

    A) Snake Family Colubridae includes almost 1400 species, or 75% of all the snake genera and 78% of all the snake species in the world (McKinstry, 1978). This topic is limited to colubrids from the New World (North, Central, and South America) only, which are considered nonvenomous.

Specific Substances

    1) Ahaetulla papuae
    2) Amphiesma stolata
    3) Boiga dendrophia
    4) Boiga irregularis (Brown Treesnake)
    5) Boiruna maculata (Clelia occiptolutea)
    6) Coelognathus radiatus (Asian ratsnake; or Elaphe radiate)
    7) Clelia clelia plubea (South American Opisthoglyph)
    8) Coluber rhodorachis (Jan's Desert Racer)
    9) Diadophis punctatus
    10) Heterodon nasicus (Western Hognose Snake)
    11) Heterodon platyrhinos (Eastern Hognose Snake)
    12) Hydrodynastes gigas
    13) Leptophis ahaetulla (Parrot Snake)
    14) Philodryas olfersii
    15) Philodryas patageniensis
    16) Stenorrhina freminvillei (Mexico)
    17) Tantilla nigriceps
    18) Thamnodynastes pallidus
    19) Thamnophis elegans vagrans (Wandering Garter Snake)
    20) Thamnophis s. sirtalis (Eastern Garter Snake)
    21) Trimorphodon biscutatus
    22) West Indian Racers
    23) COLUBRID, NEW WORLD
    24) COLUBRIDAE, NONVENOMOUS SNAKES
    25) COLUBRIDS, NONVENOMOUS SNAKES
    26) NONVENOMOUS COLUBRIDS
    27) Queen snake
    28) King snake
    29) Milk snake
    30) Corn snake
    31) Bull snake
    32) Fox snake
    33) Rat snake
    34) Garter snake
    35) Hognose snake
    36) Indigo snake
    37) Mussurana
    38) (de Araujo & dos Santos, 1997; Johnson, 1988; Hayes & Hayes, 1985; Minton, 1979)

Life Support

    A) This overview assumes that basic life support measures have been instituted.

Clinical Effects

    0.2.1) SUMMARY OF EXPOSURE
    A) WITH POISONING/EXPOSURE
    1) These snakes are generally considered nonvenomous but some select species have produced local and mild systemic envenomation. Various factors, such as the small size of the typical colubrid snake's mouth, mid- to rear location of fangs, and an inefficient venom delivery apparatus, tend to decrease the likelihood of human envenomation. A larger snake or smaller body part size, and longer duration of bite contact increases the likelihood of envenomation.
    2) Clinical effects with New World colubrids are generally mild and localized and consist of pain, edema, erythema, ecchymosis and numbness which develop rapidly and resolve over one to two weeks. Excessive salivation with metallic taste, and severe headache have also been reported. No fatalities from New World colubrids have been reported.
    0.2.14) DERMATOLOGIC
    A) Following nonvenomous colubrid bites, the victim may experience localized edema, erythema, pain and ecchymosis, followed by numbness, usually within 15 minutes.

Laboratory Monitoring

    A) No specific lab work (CBC, electrolytes, urinalysis) is needed unless otherwise clinically indicated.
    B) Monitor for signs of infection.

Treatment Overview

    0.4.5) DERMAL EXPOSURE
    A) OVERVIEW
    1) Wash bite area with soap and water.
    2) Administer tetanus toxoid when appropriate.
    3) Administer antibiotics if evidence of infection develops, but NOT prophylactically.

Range Of Toxicity

    A) Snake salivary components of New World colubrids are of a low order of toxicity. No fatalities have been reported.

Summary Of Exposure

    A) WITH POISONING/EXPOSURE
    1) These snakes are generally considered nonvenomous but some select species have produced local and mild systemic envenomation. Various factors, such as the small size of the typical colubrid snake's mouth, mid- to rear location of fangs, and an inefficient venom delivery apparatus, tend to decrease the likelihood of human envenomation. A larger snake or smaller body part size, and longer duration of bite contact increases the likelihood of envenomation.
    2) Clinical effects with New World colubrids are generally mild and localized and consist of pain, edema, erythema, ecchymosis and numbness which develop rapidly and resolve over one to two weeks. Excessive salivation with metallic taste, and severe headache have also been reported. No fatalities from New World colubrids have been reported.

Neurologic

    3.7.2) CLINICAL EFFECTS
    A) HEADACHE
    1) WITH POISONING/EXPOSURE
    a) CASE REPORT - A 29-year-old herpetologist presented to the hospital with fang marks, bleeding, edema, pain, warmth, and ecchymotic lesions after being bitten in the middle finger of the left hand by a male neotropical opistoglyphous colubrid Thamnodynastes cf. pallidus Linne. He also developed excessive salivation with metallic flavor, and severe headache. Following supportive care, his symptoms improved without further sequelae (Diaz et al, 2004).

Gastrointestinal

    3.8.2) CLINICAL EFFECTS
    A) EXCESSIVE SALIVATION
    1) WITH POISONING/EXPOSURE
    a) CASE REPORT - A 29-year-old herpetologist presented to the hospital with fang marks, bleeding, edema, pain, warmth, and ecchymotic lesions after being bitten in the middle finger of the left hand by a male neotropical opistoglyphous colubrid Thamnodynastes cf. pallidus Linne. He also developed excessive salivation with metallic flavor, and severe headache. Following supportive care, his symptoms improved without further sequelae (Diaz et al, 2004).

Dermatologic

    3.14.1) SUMMARY
    A) Following nonvenomous colubrid bites, the victim may experience localized edema, erythema, pain and ecchymosis, followed by numbness, usually within 15 minutes.
    3.14.2) CLINICAL EFFECTS
    A) EDEMA
    1) WITH POISONING/EXPOSURE
    a) CASE REPORT - Localized edema, erythema, pain, numbness and ecchymosis are relatively common, and have been documented in a number of case reports Boiruna maculata, Philodryas olfersii, P. patagoniensis and Thamnodynastes pallidus (dosSantos-Costa et al, 2000; deAraujo & dosSantos, 1997; dosSantos-Costa et al, 2000; Diaz et al, 2004; de Araujo & dos Santos, 1997; Silveira & Nishioka, 1992; Nishioka & Silveira, 1994; Gomez et al, 1994; Hayes & Hayes, 1985)
    b) INCIDENCE - A review of 43 Brazilian patients who had suffered bites from the colubrid snake Philodryas olfersii ("Green Snake"), found that the most common clinical finding was local pain (37.2%), swelling (34.9%), erythema (18.6%), and ecchymosis (9.3%) (Ribeiro et al, 1999).
    c) DURATION - Swelling may last one to two weeks (de Araujo & dos Santos, 1997).
    d) CASE REPORTS/ADULTS
    1) A 29-year-old field guide was bitten by a Thamnodynastes pallidus in the wild, with the snake remaining attached for 40 seconds. He developed edema and pain radiating to the entire extremity, along with ecchymotic lesions, and tactile warmth of the bitten finger. The edema reached its maximum extension 35 minutes following the bite. He also developed excessive salivation with a metallic taste, and a severe headache, but remained hemodynamically stable. Treatment included local application of ice, hydrocortisone and acetaminophen. The edema resolved within 36 hours (Diaz et al, 2004a).
    2) Two technicians working with Philodryuas olfersii were bitten in the course of their work. The snakes were attached for 15 seconds and 2 minutes, respectively. Both patients developed progressive edema, and in the first case subcutaneous ecchymosis. Vital signs and systemic coagulation tests remained normal. The edema progressed for up to 72 hours, reaching the axilla from a finger bite in the patient with the prolonged attachment. After the initial period, the edema was accompanied by pain and restricted movement. Both patients had resolution of edema over a two-week period without permanent sequelae (de Araujo & dos Santos, 1997).
    3) An 18-year-old male was bitten on the interphalangeal joint of the thumb by a Stenrrhina feminvillei, which attached for 45 seconds before being forcibly removed. Immediate pain developed and swelling occurred within 20 minutes. Edema spread to the wrist and first three fingers and resolved over a three-day period, with residual soreness lasting approximately a month (Cook, 1984).
    4) A man was bitten by a large "Eastern Garter Snake" (Thamnophis sirtalis sirtalis), at the base of the right index finger. Swelling of the hand developed immediately, and increased over the next 3 hours. The next morning the hand was cold and bluish in color. Lymphadenitis occurred involving the axillary and supratrochlear nodes (Hayes & Hayes, 1985a).
    e) CASE REPORTS/PEDIATRIC
    1) A 16-year-old boy was bitten by a Wandering Garter snake (Thamnophis elegans vagrans) and experienced pain and swelling of the bitten finger within 5 minutes. Edema progressed to the dorsum of the hand and wrist after 6 hours. Within 24 hours, ecchymotic areas and hemorrhagic vesicles were present locally. All laboratory values remained with normal limits. Treatment consisted of local wound care, tetanus toxoid, and prophylactic antibiotics. Full recovery occurred by day 7 (Gomez et al, 1994).
    2) An 8-year-old boy, was bitten on the thumb by a large "Wandering Garter Snake" (Thamnophis elegans vagrans). Within 5 minutes, swelling around the puncture wounds began. Three hours post bite, the hand was edematous and swollen to the wrist, and ecchymosis of the tissues was noted. Four hours post bite, the victim reported "soreness" of the hand, the arm became hypersensitive to movement, and flexing of the fingers proved very painful. Recovery was uneventful, and symptoms had completely resolved by the seventh day post bite (Vest, 1981).
    3) A 13-year-old boy was bitten at the base of the index finger by an eastern garter snake (Thamnophis s. sirtalis) with prolonged attachment, and developed coolness, edema, and ecchymosis of the bitten hand over the next 3 hours. Lymphadenitis and tender/swollen axillary nodes also occurred. There were no systemic symptoms, all laboratory tests were normal and recovery followed within 24 hours. Subsequent bites to this subject, suggested that this was an envenomation, rather than an allergic response (Hayes & Hayes, 1985)
    4) A 16-month-old girl was bitten on the left ankle while sleeping in her cradle at night. The snake was positively identified as a young male Boiruna maculata. Shortly after the bite, a tourniquet was placed above the knee, and was removed on arrival at the hospital. Fang marks were seen at the ankle, as well as edema, local erythema and cyanosis. Clotting time was normal. The patient was discharged without treatment. Seven hours later, she was readmitted with pronounced edema and pain, ecchymotic lesions, enlarged inguinal lymph nodes, cyanosis of the ankle and foot and warmth of the bitten limb. Vital signs, clotting time, and urinalysis were within normal limits. No systemic effects were observed. The patient was given 200 mL of Bothrops antivenom IV (Instituto Butantan), along with analgesics, elevation, and hydration. By the third day, the pain and cyanosis started decreasing. On the fifth day, the edema and ecchymosis were decreased. The patient was discharged on day 6 with only mild ankle edema and ecchymotic lesions (dosSantos-Costa et al, 2000).
    5) A 5-year-old boy was bitten by the Colubrid snake Philodryas patagoniensis, and developed swelling and warmth in the affected limb (Nishioka & Silveira, 1994).

Monitoring Parameters Levels

    4.1.1) SUMMARY
    A) No specific lab work (CBC, electrolytes, urinalysis) is needed unless otherwise clinically indicated.
    B) Monitor for signs of infection.

Radiographic Studies

    A) RADIOGRAPHIC-OTHER
    1) For bitten areas that remain tender, plain radiographs may reveal the presence of embedded snake tooth fragments (Weed, 1993).

Life Support

    A) Support respiratory and cardiovascular function.

Patient Disposition

    6.3.6) DISPOSITION/BITE-STING EXPOSURE
    6.3.6.1) ADMISSION CRITERIA/BITE-STING
    A) GENERAL
    1) Hospitalization is unnecessary, except in rare cases where the patient is exhibiting severe symptoms.
    B) DISCHARGE CONSIDERATIONS
    1) If a patients requires hospitalization, pain control may be necessary after discharge. Infection or inflammatory changes similar to infection may occur and should be managed with antibiotics as needed.
    6.3.6.3) CONSULT CRITERIA/BITE-STING
    A) In the US, envenomations are likely to occur in zoos, or academic or private collector settings. Snake identification may be inaccurate in non-institutional settings. Obtaining an accurate identification of the snake is of utmost importance in order to guide treatment and monitoring. When dealing with private collectors, consideration should be given to independently verify the snake species. A regional poison center can assist with envenomation management, and should be contacted whenever a patient develops unexpected symptoms after being bitten by a supposedly nonvenomous snake. A local zoo or aquarium may be of assistance in identifying the snake.
    B) Information found on the Online Antivenom Index (www.aza.org/AI/Index) can be provided by a regional poison center by contacting (1-800-222-1212).
    C) Consult with a surgeon if necrotic areas develop.

Monitoring

    A) No specific lab work (CBC, electrolytes, urinalysis) is needed unless otherwise clinically indicated.
    B) Monitor for signs of infection.

Dermal Exposure

    6.9.1) DECONTAMINATION
    A) DERMAL DECONTAMINATION
    1) Thoroughly wash the bitten area with soap and water.

Summary

    A) Snake salivary components of New World colubrids are of a low order of toxicity. No fatalities have been reported.

Minimum Lethal Exposure

    A) GENERAL/SUMMARY
    1) Bites from nonvenomous New World colubrid have NOT resulted in fatality, but may produce mild local and rarely systemic symptoms.

Maximum Tolerated Exposure

    A) GENERAL/SUMMARY
    1) These snakes are generally considered nonvenomous but some select species have produced local and mild systemic envenomation. Although many are too small to routinely cause envenomation in humans, this can occur with larger snakes especially in smaller individuals or a specific body part.

Toxicologic Mechanism

    A) The precise pharmacologic/toxicologic mechanisms of the salivary components is not completely understood.
    B) Most of the venom of colubrid snakes is secreted by the Duvernoy's (parotid) gland, found in the upper lip or at the angle of the mouth.
    C) Some symptoms could be due to individual sensitivity to salivary proteins.
    D) The suspected enzymes responsible for local injury are metalloproteases, described for P. olfersii and Thamnodynastes strigilis, with the proteolytic degradation of basement membrane components of capillary vessels producing local hemorrhage and edema (Diaz et al, 2004a).
    E) The venom of Philodryas olfersii ("Green Snake"), has strong activities in producing the following: hemorrhage, edema, and fibrinogenolysis (Assakura et al, 1992).
    F) Venom from 12 species of colubrid snakes showed toxic components with proteolytic activity, low phosphodiesterase activity in several venoms (Amphiesma stolata, Diadophis punctatus, Heterodon nasicus kennerlyi, H. n. nasicus and Thamnophis elegans vagrans), and acetylcholinesterase in Boiga irregularis saliva and venom. High phospholipase A(2) (PLA(2)) activity was found in Trimorphodon biscutatus lambda venom, and moderate levels were detected in Boiga dendrophila and D. p. regalis venoms, as well as B. dendrophila and H. n. nasicus salivas. Components from A. stolata, Hydrodynastes gigas, Tantilla nigriceps and T. e. vagrans venoms showed protease activity (Hill & Mackessy, 2000).
    G) Durvenoy's gland secretion of Philodryas patagoniensis exhibited a potent fibrinogenolytic activity degrading the alpha-chain faster than the beta-chain, whereas gamma-chain was resistant in human plasma and in vivo rat models. The secretion also hydrolyzed fibrin degrading the alpha-monomer. Metalloproteinases and serinoproteinases are the main enzymes responsible for the hydrolyzing activity on fibrinogen and fibrin. These hydrolyzing activities and those acting on the wall of blood vessels let the secretion exhibit a high hemorrhagic activity (Peichoto et al, 2005).
    H) Durvenoy's gland secretion of Philodryas patagoniensis showed a high edematogenic activity and moderate myonecrotic and dermonecrotic activities, while lacking phospholipase A(2) effect in a mouse footpad injection model. Microscopically, myonecrosis reached its highest intensity 12 hours after injection, which was also demonstrated by serum creatine kinase levels. Dermonecrosis was proportional to the amount of secretion injected. The enzymes responsible for those activities are mostly metalloproeinases (Peichoto et al, 2004).
    I) A toxin isolated from the venom of Boiga dendrophia (mangrove catsnake) displayed potent postsynaptic neuromuscular activity and irreversibly inhibited indirectly stimulated twitches in nerve-muscle preparations. This activity is 100-fold greater in birds, their natural prey, than in mice (Pawlak et al, 2006).
    J) The venom of Boiga dendrophila was found to have muscarinic and purinergic (adenosine-like) receptor effects in a rat muscle preparation (Lumsden et al, 2004).
    K) Rats injected with venom from Phiodryas patagoniensis demonstrated abnormal liver transaminase levels, and histopathologic changes in a variety of organs, including multifocal hemorrhage in the cerebellum, cerebrum and lung as well as severe peritubular capillary congestion in kidney and hydropic degeneration in the liver (Peichoto et al, 2004).
    L) The chick muscle acetylcholine receptor was 100-fold more susceptible to the neurotoxin of Boiga dendrophila compared with the mouse receptor, indicating bird-specific postsynaptic activity (Pawlak et al, 2006).
    M) Two enzymes, azocaseinolytic metalloprotease and acetylcholinesterase, were detected in venom from Boiga irregularis. Adult B. irregularis venom was very toxic to domestic chickens and lizards, characterized by rapid paralysis. High toxicity toward non-mammalian prey demonstrated the presence of taxa-specific effects (and thus toxins) in B. irregularis venom. The authors hypothesize that the lack of significant envenomation effects in humans following most colubrid bites results from this taxa-specific action of colubrid venom components, not from a lack of toxins (Mackessy et al, 2006).
    N) Duvernov's gland secretions from Thamnodynastes stigilis showed proteolytic hemorrhagic, and neurotoxic activities in chicken embryo and mouse skin and peritoneum assays (Lemoine et al, 2004).

Clinical Effects

    11.1.13) OTHER
    A) OTHER
    1) Clinical effects in animals are expected to be of the same nature as with human victims.

Treatment

    11.2.1) SUMMARY
    A) GENERAL TREATMENT
    1) Veterinary treatment is the same as with human victims.

Continuing Care

    11.4.1) SUMMARY
    11.4.1.2) DECONTAMINATION/TREATMENT
    A) GENERAL TREATMENT
    1) Veterinary treatment is the same as with human victims.

General Bibliography

    1) Assakura MT, Da Graca Salomao M, & Puorto G: Hemorrhagic, fibrinogenolytic and edema-forming activities of the venom of the colubrid snake philodryas olfersii (green snake). Toxicon 1992; 30:427-438.
    2) Cook DG: A case of envenomation by the neotropical colubrid snake, Stenorrhina freminvillei. Toxicon 1984; 22(5):823-827.
    3) Diaz F, Navarrete LF, Pefaur J, et al: Envenomation by neotropical opisthoglyphous colubrid Thamnodynastes cf. pallidus Linne, 1758 (Serpentes:Colubridae) in Venezuela. Rev Inst Med Trop Sao Paulo 2004a; 46(5):287-290.
    4) Diaz F, Navarrete LF, Pefaur J, et al: Envenomation by neotropical opistoglyphous colubrid Thamnodynastes cf. pallidus Linne, 1758 (Serpentes:Colubridae) in Venezuela. Rev Inst Med trop S Paulo 2004; 46(5):287-290.
    5) Gomez HF, Davis M, & Phillips S: Human envenomation from a wandering garter snake. Ann Emerg Med 1994; 23:1119-1122.
    6) Hayes WK & Hayes FE: Human envenomation from the bite of the Eastern Garter Snake, Thamnophis s. sirtalis (Serpentes: Colubridae). Toxicon 1985a; 23(4):719-721.
    7) Hayes WK & Hayes FE: Human envenomation from the bite of the eastern garter snake, Thamnophis s. sirtalis (Serpentes: Colubridae). Toxicon 1985; 23(4):719-721.
    8) Hill RE & Mackessy SP: Characterization of venom (Duvernoy's secretion) from twelve species of colubrid snakes and partial sequence of four venom proteins. Toxicon 2000; 38(12):1663-1687.
    9) Johnson JJ: Comments on the report of envenomation by the Colubrid snake Sternorrhina freminvillei - Letter to the editor. Toxicon 1988; 26(6):519-521.
    10) Kasilo OM & Nhachi CF: A retrospective study of poisoning due to snake venom in Zimbabwe. Hum Exp Toxicol 1993; 12(1):15-18.
    11) Lemoine K, Salgueiro LM, Rodriguez-Acosta A, et al: Neurotoxic, hemorrhagic and proteolytic activities of Duvernoy's gland secretion from Venezuelan opisthoglyphous colubrid snakes in mice. Vet Hum Toxicol 2004; 46(1):10-14.
    12) Lumsden NG, Fry BG, Manjunatha KR, et al: In vitro neuromuscular activity of 'colubrid' venoms: clinical and evolutionary implications. Toxicon 2004; 43(7):819-827.
    13) Mackessy SP, Sixberry NM, Heyborne WH, et al: Venom of the Brown Treesnake, Boiga irregularis: ontogenetic shifts and taxa-specific toxicityMACKESSY2006. Toxicon 2006; 47(5):537-548.
    14) McKinstry DM: Evidence of toxic saliva in some Colubrid snakes of the United States. Toxicon 1978; 16:523-534.
    15) Minton SA: Beware: nonpoisonous snakes. Clinical Toxicology 1979; 15(3):259-265.
    16) Nishioka SA & Silveira PVP: Philodryas patagoniensis bite and local envenoming. Rev Inst Med Trop Sao Paulo 1994; 36(3):279-281.
    17) Pawlak J, Mackessy SP, Fry BG, et al: Denmotoxin, a three-finger toxin from the colubrid snake Boiga dendrophila (Mangrove Catsnake) with bird-specific activity. J Biol Chem 2006; 281(39):29030-29041.
    18) Peichoto ME, Acosta O, Leiva L, et al: Muscle and skin necrotizing and edema-forming activities of Duvernoy's gland secretion of the xenodontine colubrid snake Philodryas patagoniensis from the north-east of ArgentinaPEICHOTO2004. Toxicon 2004; 44(6):589-596.
    19) Peichoto ME, Leiva LC, GuaimasMoya LE, et al: Duvernoy's gland secretion of Philodryas patagoniensis from the northeast of Argentina: its effects on blood coagulationPEICHOTO2005. Toxicon 2005; 45(4):527-534.
    20) Ribeiro LA, Puorto G, & Jorge MT: Bites by the colubrid snake philodryas olfersii: a clinical and epidemiological study of 43 cases. Toxicon 1999; 37:943-948.
    21) Silveira PVP & Nishioka SA: Non-vneomous snake bite and snake bite without envenoming in a Brazilian teaching hospital. Analysis of 91 cases. Rev Inst Med Trop Sao Paulo 1992; 34:499-503.
    22) Vest DK: Envenomation following the bite of a Wandering Garter Snake (Thamnophis elegans vagrans). Clinical Toxicology 1981; 18(5):573-579.
    23) White J: Clinical Toxicology of sea snake bites In: Meier J & White J (Eds): Handbook of Clinical Toxicology of Animal Venoms and Poisons, CRC Press, New York, NY, USA, 1995, pp 159-170.
    24) de Araujo ME & dos Santos AC: Cases of human envenoming caused by Philodryas olfersii and Philodryyas patagoniensis (Serpentes: Coulubridae). Rev Soc Bras Med Trop 1997; 30:517-519.
    25) deAraujo ME & dosSantos AC: Cases of human envenoming caused by Philodryas olfersii and Philodryas patagoniensis (Serpentes: Colubridae). Rev Soc Bras Med Trop 1997; 30(6):517-519.
    26) dosSantos-Costa MC, Outeiral AB, & et al: Envenomation by the neotropical colubrid Boiruna maculata (Boulenger, 1896): a case report. Rev Inst Med Trop Sao Paulo 2000; 42(5):283-286.